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The Human Epigenetic Drug Database (HEDD) is a comprehensive web-based database for epigenetic drugs, which focuses on the storage and integration of epigenetic drug datasets that were obtained from laboratory experiments that is essential for understanding the mechanism of action of these epigenetic drugs at a systematic level.

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Gene expression changes upon Dot1L knockdown and Dot1L inhibitor treatment during reprogramming

Drug Name:EPZ004777
Drug Category:HMTi
Sample Type:Embryonic stem cell-derived fibroblasts (dH1fs)
Condition:healthy
Experiment Type:Expression profiling by array
Experiment Platform:GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array GPL3921 [HT_HG-U133A] Affymetrix HT Human Genome U133A Array
Description:Through a loss-of-function approach, we identified that inhibition of the histone methyltransferase, Dot1L, accelerated somatic cell reprogramming, significantly increased the yield of induced pluripotent stem (iPS) cell colonies, and substituted for Klf4 and c-Myc in the reprogramming cocktail. To understand the mechanism by which Dot1L inhibition results in these phenotypes, we carried out gene expression profiling using Affymetrix microarrays. GSM880675-GSM880682: dH1fs were treated with 10uM Dot1L inhibitor (EPZ004777) and then superinfected with Oct4, Sox2, Klf4 and c-Myc (OSKM) retroviruses. Total RNA was harvested 6 days later. There were two biologic replicates for each condition.
PubmedID:22388813
Citation:Onder TT;Kara N;Cherry A;Sinha AU;Zhu N;Bernt KM;Cahan P;Marcarci BO;Unternaehrer J;Gupta PB;Lander ES;Armstrong SA;Daley GQ. Chromatin-modifying enzymes as modulators of reprogramming. Nature 2012 Mar 4;483(7391):598-602.
Downlaod:File Download (206.055MB)

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The Human Epigenetic Drug Database (HEDD) is a comprehensive web-based database for epigenetic drugs, which focuses on integrating epigenetic drug studies based Omics data from high through experiments that is essential for understanding the mechanism of action of these epigenetic drugs at a systematic level.

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©2016 Group of Computational Epigenomics and Bioinformatics, College of Life Science, Jilin Normal University, China

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