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The Human Epigenetic Drug Database (HEDD) is a comprehensive web-based database for epigenetic drugs, which focuses on the storage and integration of epigenetic drug datasets that were obtained from laboratory experiments that is essential for understanding the mechanism of action of these epigenetic drugs at a systematic level.

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A multiplexed system for quantitative comparisons of chromatin landscapes

Drug Name:C646;GSK126;KDM5-C70
Drug Category:HATi;HMTi ;KDMi
Sample Type:K562 cells SKM-1 and Toledo cells Human HSCs
Condition:healthy
Experiment Type:Genome binding/occupancy profiling by high throughput sequencing
Experiment Platform:GPL18573 Illumina NextSeq 500 (Homo sapiens) GPL19057 Illumina NextSeq 500 (Mus musculus)
Description:Genome-wide profiling of histone modifications can provide systematic insight into the regulatory elements and programs engaged in a given cell type. However, conventional chromatin immunoprecipitation and sequencing (ChIP-seq) does not capture quantitative information on histone modification levels, requires large amounts of starting material, and involves tedious processing of each individual sample. Here we address these limitations with a technology that leverages DNA barcoding to profile chromatin quantitatively and in multiplexed format. We concurrently map relative levels of multiple histone modifications across multiple samples, each comprising as few as a thousand cells. We demonstrate the technology by monitoring dynamic changes following inhibition of P300, EZH2 or KDM5, by linking altered epigenetic landscapes to chromatin regulator mutations, and by mapping active and repressive marks in purified human hematopoietic stem cells. Hence, this technology enables quantitative studies of chromatin state dynamics across rare cell types, genotypes, environmental conditions and drug treatments.
PubmedID:26687680
Citation:van Galen P;Viny AD2;Ram O1;Ryan RJ1;Cotton MJ;Donohue L; Sievers C;Drier Y;Liau BB;Gillespie SM; Carroll KM;Cross MB; Levine RL; Bernstein BE.A Multiplexed System for Quantitative Comparisons of Chromatin Landscapes. Mol Cell 2016 Jan 7;61(1):170-80.
Downlaod:Relative Link

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The Human Epigenetic Drug Database (HEDD) is a comprehensive web-based database for epigenetic drugs, which focuses on integrating epigenetic drug studies based Omics data from high through experiments that is essential for understanding the mechanism of action of these epigenetic drugs at a systematic level.

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©2016 Group of Computational Epigenomics and Bioinformatics, College of Life Science, Jilin Normal University, China

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